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KMID : 1377020180150030311
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Tissue Engineering and Regenerative Medicine
2018 Volume.15 No. 3 p.311 ~ p.319
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Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography
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Jin Long Hao
Choi Byung-Hyune
Kim Young-Jick
Oh Hyun-Ju
Kim Byoung-Ju
Yin Xiang Yun
Min Byoung-Hyun
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Abstract
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It is very useful to evaluate the content and 3D distribution of extracellular matrix non-destructively in tissue engineering. This study evaluated the feasibility of using micro-computed tomography (μCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffolds in vitro. Engineered cartilages were incubated with Hexabrix 320 for 20 min and analyzed via μCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D μCT images of an engineered cartilage showed positive correlation with the histological image of Safranin-O staining. Quantitative data obtained with the 3D μCT images of 14 engineered cartilages showed strong correlation with sulfated GAGs contents obtained by biochemical analysis (R2?=?0.883, p?
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KEYWORD
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Micro-CT (μCT), Glycosaminoglycans (GAGs), Hexabrix 320, Cartilage, Tissue engineering
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